Introduction to Genetic Engineering – Bacterial Transformation
Bacterial Transformation Lab Introduction
In this lesson, students explore genetic engineering through bacterial transformation of a gene from bioluminescent jellyfish resulting in bright green fluorescent bacterial colonies producing the Green Fluorescent Protein (GFP). Transformation is used widely in biotechnology ranging from the production of human insulin to introducing genes coding for traits such as frost, pest or drought resistance in plants.
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Lesson Resources
Student Guide – Download and edit student worksheets to be completed during the Bacterial Transformation Lab.
Lesson Slides – Articulates with the student guide above and accompanying lesson plans below.
Lesson Plan – Suggested times for pacing, sequence and activities. Includes vocabulary tool, student reading, and other lesson resources.
Supplemental Resources – Includes background information, outside articles, videos, and more related to the lab.
Lab Resources
Student Lab Protocol – Students follow these instructions when doing the Bacterial Transformation Lab.
Preparing the Classroom for the Lab – How to set up group stations for this lab experience.
Inventory Guide – Reagents and materials checklist for one classroom kit. Includes equipment and classroom needs for the lab.