Curriculum

BABEC supports the following curriculum with teacher training and portable biotech labs. Our curriculum meets and, in some cases, exceeds the recommendations of the National Science Education Standards and the State of California Science Standards. In our classes, students model the scientific method: they hypothesize, experiment, collect, and analyze their data, and support their conclusions with evidence, all in the context of contemporary issues in biotechnology.

Polymerase Chain Reaction (PCR) Lab Activities

PCR

Introduction to PCR
See how PCR is simply DNA replication in a test tube
Students to take a closer look at each of the the individual components in the reaction and the theory behind the technology.
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Alu PCR
Analyze a genetic polymorphism to study human populations

Students learn about allele frequencies, transposable DNA elements, and issues of identity and privacy.
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Forensic PCR
Learn a technique for forensic analysis of DNA samples

Students determine their individual “DNA fingerprint” and learn about subtle variations in the human genome.
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Tracing your Matrilineal Ancestry:
Mitochondrial DNA PCR, Sequencing and Bioinformatics
Find out where you’re really from
Students discover their deep ancestry and learn how we all connect on the human family tree.
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GMO PCR
Understand the science and make informed decisions

Students are introduced to the process of genetic engineering and the detection of transgenic DNA in our food supply.
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PCR Optimization
Explore how negligible changes can greatly influence experimental outcomes 

Students optimize PCR components to learn how scientists design and improve experiments through trial and error.
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Wolbachia PCR
Discover the microbes within!

Students learn how lateral gene transfer between species, symbiosis, and co-evolution describe our world.
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Genetic Engineering Lab Activities

fluorescent-proteins-blue

Bacterial Transformation with GFP
Students transform bacteria with a plasmid containing a gene for a green fluorescent protein

By transforming cells with a plasmid containing a gene for green fluorescent protein, students understand that changing an organism’s genotype can result in a remarkably different phenotype.
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Purification of GFP
Students isolate the green fluorescent protein from a mixture of proteins using nickel affinity chromatography
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Polyacrylamide Gel Electrophoresis (PAGE) of Purified GFP
Students analyze the purified protein on a denaturing gel
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gretchen@18east.comCurriculum