By transforming cells with a plasmid containing a gene for green fluorescent protein, students understand that changing an organism’s genotype can result in a remarkably different phenotype. Students insert a jellyfish gene into laboratory-safe bacteria and induce production of a green fluorescent protein. Students understand the power of this technology and discuss the economic and social ramifications of recombinant DNA technology. This activity will allow you to teach about gene regulation and transcriptional activation. The GFP plasmid makes a neon green fluorescent protein that can be seen under UV light.
Beginner Lab: This lesson is appropriate for any biology or biotechnology class.
pGLO – Bacterial Transformation Student Laboratory Guide
pKiwi – Bacterial Transformation Student Laboratory Guide
For teacher guides, and new information about how to incorporate NGSS, click HERE.
Coming soon…Rainbow Plasmids!
In fall 2017 we will offer a new array of plasmids containing genes for four new colors. These will allow for extension activities like protein purification with affinity chromatography and SDS-PAGE analysis. These plasmid make colorful proteins that are visible by eye in transformed cells. Stay tuned.