Micropipetting – Measuring and Moving Small Volumes

Micropipetting – Measuring and Moving Small Volumes
This lesson is the second of the “Getting Started” series which includes DNA extraction, Micropipetting, and gel electrophoresis. This series introduces students to concepts and lab fundamentals that may prepare them to perform more advanced PCR labs including DNA extraction from their own cheek cells, insects (Wolbachia PCR) or food material (GMO PCR).
Read More

    Micropipettes are tools used for accurately and precisely transferring small volumes. Micropipetting in a fundamental skill used by molecular biologists to carry out experiments. While this lesson is primarily designed to precede units on biotechnology, it could also be utilized as part of a unit on metric measurement and scale.

    In this lesson, students will engage in activities to teach them the proper way to use a micropipette and familiarize themselves with commonly used metric units and measurements. Students will use X-Y coordinates and colored liquids to generate pipette art and test their precision by pipetting specified volumes. Additional lab activities including “Roy, Gee, and Biv’s Challenge” are linked under Supplemental Materials in the lesson plan.

Lesson Resources
Student Guide – This guide articulates with Micropipette Art and Precision Pipetting activities. For additional lab activities see Supplemental Resources in the Lesson Plan.
Lesson Slides – Articulates with the student guide above and accompanying lesson plans below.
Lesson Plan – Suggested times for pacing, sequence and activities for the lab. Includes vocabulary tool, supplemental resources, etc.

Lab Resources
Student Lab Protocol – Student instructions for Micropipetting activities are embedded in the Student Guide and Lesson Plan
Preparing the Classroom for the Lab – How to set up group stations for this lab experience.
Inventory Guide – Reagents and materials checklist for one classroom kit. Includes equipment and classroom needs for the lab.

– Accurately and precisely measure small
volumes, in metric units of microliter, using
a micropipette

Crosscutting Concepts:
– Scale, Proportion, and Quantity
Micropipettes are a useful tool for helping
students visualize very small volumes and
recognize proportional relationships between
different quantities on a metric scale.

Familiarity with the following concepts may be helpful for students prior to using the lesson resources.
– Metric scale for volumetric units (liter,
milliliter, and microliter)
– Usefulness of working with small volumes
in a forensics or basic research setting
Kitty MeiMicropipetting – Measuring and Moving Small Volumes