Micropipetting II: Setting the Volume

This lesson follows “Micropipetting I: Carbonation Investigation.” In this one to two day lesson, students complete their micropipetting training by learning how to read and set the volume of P20, P200, and P1000 micropipettes. In the lab portion, they first test their precision by pipetting specified volumes onto a laminated sheet. They then deepen their practice by completing “Roy, Gee, and Biv’s Challenge” to create a mystery pattern by following specific steps to transfer red, yellow, and blue dyes.

In the online version of this lesson, students learn about micropipetting by engaging with a series of videos and simulations and practice reading the volume settings on different sized micropipettes.

Learning Objectives

  • Read and set the volume of P20, P200, and P1000 micropipettes
  • Accurately and precisely measure small volumes using micropipettes

Prior Knowledge

  • Mechanics of using a micropipette (see “Micropipetting I: Carbonation Investigation”)
  • Metric scale for volumetric units (liter, milliliter, and microliter)







In order to access any Curriculum resource, please or sign up if you haven't already done so.

Crosscutting Concepts:

Scale, Proportion and Quantity

In order to access any Curriculum resource, please or sign up if you haven't already done so.

Amanda Cherry
Paradise Valley HS
Phoenix, AZ

The curriculum materials are beautifully prepared and easy to understand-thanks for preparing such great resources that are easy to use.

Byron Gougoumis
Burton HS
San Francisco, CA

All Babec labs are well organised and modelled effectively with clear, helpful procedural instructions, easily transposed to the classroom.

Gianne Souza
Lincoln HS
San Francisco, CA

The online resources are SO well done! The best I have ever had. Very little modification to make them clear and easy to follow. I have done two BABEC lessons and they are so well made that they made teaching online fun again.

Heather Johnson
Burlingame HS
Burlingame, CA

When doing the BABEC labs I consistently hear students say they feel like "real scientists."

Marianne Gudmundsson
Glenbrook South HS
Glenview, IL

Well organized and scaffolded; flexible structure and can be used partially or as a whole. This unit can be used as is...superbly written and thought out...incorporates many skills and allows student collaboration and student choice/voice.

Rocky Ng
South San Francisco HS
South San Francisco, CA

Thank you for creating these curriculum materials. I am beyond grateful for the quality lessons, on-the-point figures/videos, and easy-to-navigate biotechnology curriculum.

Thi Ngo
Capuchino HS
San Bruno, CA

The level of support really made the whole process easier and encouraged me to continue incorporating biotech into my curriculum. Thank you for the live assistance during the labs and for responding to inquiries so promptly and with such urgency.

Anonymous Teacher

Really well thought out curricula. Great to see "old" activities that have been re-imagined with lots of consideration to NGSS.

Anonymous Teacher

This was the best educational workshop I have had the honor of attending, and I am so thankful.

Anonymous Teacher

Wonderfully executed. Strengthened my skills. Thank you!!

Anonymous Teacher

BABEC has such a strong positive impact on teachers and students - thank you for your amazing work!